maternal-effect gene expression in cultured preantral follicles derived from vitrified-warmed mouse ovary
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Maternal-Effect Gene Expression in Cultured Preantral Follicles Derived from Vitrified-Warmed Mouse Ovary
OBJECTIVE This study was conducted to assess survival of follicles, their oocyte maturation and fertilization potential as well as expression of early embryo developmental genes in in vitro cultured pre-antral follicles derived from vitrified-warmed mouse ovary. MATERIALS AND METHODS In this experimental study, ovaries of 12-day old Naval Medical Research Institute (NMRI) female mice were pla...
متن کاملSurvival Rate of Preantral Follicles Derived From Vitrified Mouse Ovarian Tissue by Cryotop
Purpose: The aim of this study was to investigate the growth and survival rate of preantral follicles isolated from vitrified ovarian tissue by cryotop.Materials and Methods: Ovaries of 14-day-old NMRI mice were separated and divided into three groups fowling as: vitrified, toxicity tested and control groups. Ovaries in the experimental groups were immersed in equilibration and vitrification so...
متن کاملP-219: GDF9, BMP15 and Their Receptors Expression During In Vitro Culture of Mouse Vitrified Ovarian Tissue Derived Preantral Follicles
Background: In vitro culture (IVC) of isolated preantral follicles from cryopreserved ovarian tissue might be an efficient method for enhancing mature oocytes in patients who are exposed to infertility. Materials and Methods: Ovaries of 13-day old NMRI mice were removed and randomly placed into control,needle immersed (NIV) and solid surface vitrification (SSV) groups. For vitrification, ovarie...
متن کاملP-99: The Effects of Zinc in Vitrification Medium on In Vitro Growth of Follicles Derived from Vitrified-Warmed Mouse Ovary
Background: The development of a reliable method for the cryopreservation of mammalian ovary would be an important advancement in the field of reproductive biology for the preservation of genetic resources. The cryopreserved follicles have the potential to develop in-vitro; however, the developmental rate is lower than that of fresh follicles. Researchers have used that of different cryoprotect...
متن کاملP-116: Maturation Genes Expression during In Vitro Culture of Vitrified Mouse Preantral Follicles
Background: Evaluation of maturation genes expression and their pattern during in vitro culture of vitrified preantral follicles is an essential and could promote either vitrification procedure or culture condition. Materials and Methods: Preantral follicles (with 100- 130 μm diameter) isolated mechanically from 12-14 days old NMRI mice ovaries and divided into vitrification and control groups....
متن کاملP-160: The Effects of Vanadium in Vitrification Medium on In Vitro Growth of Follicles Derived from Vitrified-Warmed Mouse Ovary
Background: Cryopreservation of ovarian tissue is an important option in order to preserve the fertility of cancer patients undergoing chemotherapy and radiotherapy. There are many reports on adding some materials such as calcium, ascorbate and antioxidants to freezing medium. Vanadium is one of the minerals, which is very important as a trace element for normal cell function as well as develop...
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عنوان ژورنال:
cell journalجلد ۱۷، شماره ۲، صفحات ۳۳۲-۳۳۸
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